3T6-Swiss albino小鼠胚胎成纖維細(xì)胞
描述:3T6 細(xì)胞株1963年從分解的瑞士小鼠胚胎中建立,能分泌膠原質(zhì)和透明質(zhì)酸。 檢測表明肢骨發(fā)育畸形病毒(鼠痘)陰性。
動(dòng)物種別:小鼠
性別:***
形態(tài):成纖維細(xì)胞
培養(yǎng)基和添加劑:DMEM培養(yǎng)基(GIBCO,貨號(hào)12800017,添加NaHCO3 1.5g/L),90%;胎牛血清,10%。 氣相:空氣,95%;二氧化碳,5%。 溫度:37攝氏度。
供應(yīng)限制:A。僅供研究之用。
REFERENCE:Todaro GJ , Green H . Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines. J. Cell Biol. 17: 299-313, 1963. PubMed: 13985244 Vogt M , Dulbecco R . Studies on cells rendered neoplastic by polyoma virus: the problem of the presence of virus-related materials. Virology 16: 41-51, 1962. PubMed: 13926482 Green H , et al. Differentiated cell types and the regulation of collagen synthesis. Nature 212: 631-633, 1966.
中文名稱:小鼠胚胎成纖維細(xì)胞