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人正常肺上皮細(xì)胞BEAS-2B
??【編號(hào)】:BEAS-2B

??【名稱】:人正常肺上皮細(xì)胞BEAS-2B

??【規(guī)格】:株

??【價(jià)格】:

  BEAS-2B 暫不供應(yīng)人正常肺上皮細(xì)胞

  描述:從一位非癌個(gè)體的正常人支氣管上皮病理切片分離出上皮細(xì)胞。 這些細(xì)胞用腺病毒12-SV40病毒雜交病毒感染并克隆。 DEAS-2B細(xì)胞保留了對(duì)血清反應(yīng)進(jìn)行鱗關(guān)分化的能力,并有用于篩選誘導(dǎo)或影響分化及致癌的化學(xué)或生物制劑。 細(xì)胞角蛋白及SV40抗原染色陽(yáng)性。
  動(dòng)物種別:人
  性別:未知
  組織來源:肺;支氣管;正常;上皮;病毒轉(zhuǎn)化
  形態(tài):上皮細(xì)胞
  培養(yǎng)基和添加劑:LHC-9培養(yǎng)液添加0.5鈉克/毫升重組上皮細(xì)胞生長(zhǎng)因子(EGF),500鈉克/毫升氫化可的松,0.035鈉克/毫升牛垂體提取物,500mM乙醇胺,500nM 磷酸乙醇胺,0.01毫克/毫升腎上腺素,0.1鈉克/毫升retinoic酸及微量元素。注意:不要過濾EGF。 LHC-9基礎(chǔ)培養(yǎng)液可以從Biofluids, Inc., Rockville, MD(目錄號(hào)118)或Clonetics Corporation, Walkersville, MD 21793(BEBM目錄號(hào)CC3171)購(gòu)得。 調(diào)整
  供應(yīng)限制:A。僅供研究之用。
  REFERENCE:Reddel RR , et al. Immortalized human bronchial epitherial mesothelial cell lines. US Patent 4,885,238 dated Dec 5 1989 Lechner JF , LaVeck MA . A serum-free method for culturing normal human bronchial epithelial cells at clonal density. J. Tissue Culture Methods 9: 43-48, 1985. Sakamoto O , et al. Role of macrophage-stimulating protein and its receptor, RON tyrosine kinase, in ciliary motility. J. Clin. Invest. 99: 701-709, 1997. PubMed: 9045873 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Centers for Disease Control (1993), Biosafety in Microbiological and Biomedical Laboratories Human Health Service Publication No. (CDC) 93-8395. U.S. Dept. of Health and Human Services; 3rd Edition U.S. Government Printing Office Washington D.C.
  中文名稱:人正常肺上皮細(xì)胞
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