人子宮頸表皮癌細(xì)胞ME-180
??【編號】:ME-180 ??【名稱】:人子宮頸表皮癌細(xì)胞ME-180 ??【規(guī)格】:株 ??【價格】:1800 ME-180人子宮頸表皮癌細(xì)胞
描述:這株細(xì)胞來源于一個細(xì)胞集落不規(guī)則沒有顯著角質(zhì)化的侵染性鱗狀細(xì)胞癌。 單層培養(yǎng)的細(xì)胞間可以觀察到帶狀連接,也注意到有細(xì)胞質(zhì)張力絲。 1970年發(fā)現(xiàn)支原體污染并去除。 腫瘤壞死因子(TNF)α抑制ME-180的生長。 這株細(xì)胞含有人乳頭瘤病毒(HPV)DNA,與HPV-39的同源性高于HPV-18。
動物種別:人
性別:女
組織來源:宮頸表皮樣癌網(wǎng)膜轉(zhuǎn)移灶
形態(tài):上上皮細(xì)胞
培養(yǎng)基和添加劑:McCOY's 5A(SIGMA,貨號M4892,添加NaHCO3 2.2g/L),90%;優(yōu)質(zhì)胎牛血清,10%。 氣相:空氣,95%;二氧化碳,5%。 溫度:37攝氏度。
供應(yīng)限制:A。僅供研究之用。
REFERENCE:21405: Sugarman BJ, et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985. PubMed: 3933111 22520: Sykes JA, et al. Some properties of a new epithelial cell line of human origin. J. Natl. Cancer Inst. 45: 107-122, 1970. PubMed: 4317734 22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080 22995: Pater MM, Pater A. Human papillomavirus types 16 and 18 sequences in carcinoma cell lines of the cervix. Virology 145: 313-318, 1985.www.atcc360.com PubMed: 2992153 23180: Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217 23226: Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212 23238: Reuter S, et al. Characterization of a novel human papillomavirus DNA in the cervical carcinoma cell line ME180. J. Virol. 65: 5564-5568, 1991. PubMed: 1716694 23324: Scheffner M, et al. The state of the p53 and retinoblastoma genes in human cervical carcinoma cell lines. Proc. Natl. Acad. Sci. USA 88: 5523-5527, 1991. PubMed: 1648218 29988: Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105 32285: van Dijk MA, et al. A functional assay in yeas for the human estrogen receptor displays wild-type and variant estrogen receptor messenger RNAs present in breast carcinoma. Cancer Res. 57: 3478-3485, 1997.PubMed: 9270016 32299: St. Geme JW, et al. Characterization of the genetic locus encoding Haemophilus influenzae type b surface fibrils. J. Bacteriol. 178: 6281-6287, 1996.
中文名稱:人子宮頸表皮癌細(xì)胞
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