人骨肉瘤細(xì)胞
細(xì)胞名稱:U-2 OS人骨肉瘤細(xì)胞
描述:J. Ponten and E. 這株細(xì)胞(原名2T)源自15歲女孩的脛骨中等分化的肉瘤。 與WI-38共培養(yǎng)或CF檢測(cè)SV40,RSV或腺病毒時(shí)未檢測(cè)到病毒。 1972年發(fā)現(xiàn)支原體污染并去除。
動(dòng)物種別:人
性別:女
組織來源:骨;骨肉瘤
形態(tài):上皮細(xì)胞
培養(yǎng)基和添加劑:RPMI-1640(GIBCO,貨號(hào)31800022, 添加NaHCO3 1.5g/L, glucose 2.5g/L, Sodium Pyruvate 0.11g/L),85%;熱滅活馬血清,10%;優(yōu)質(zhì)胎牛血清,5%。 氣相:空氣,95%;二氧化碳,5%。 溫度:37攝氏度。
供應(yīng)限制:A。僅供研究之用。
REFERENCE:Heldin CH , et al. A human osteosarcoma cell line secretes a growth factor structurally related to a homodimer of PDGF A-chains. Nature 319: 511-514, 1986. PubMed: 3456080 Ponten J , Saksela E . Two established in vitro cell lines from human mesenchymal tumours. Int. J. Cancer 2: 434-447, 1967. PubMed: 6081590 Raile K , et al. Human osteosarcoma (U-2 OS) cells express both insulin-like growth factor-I (IGF-I) receptors and insulin-like growth factor-II/mannose-6- phosphate (IGF-II/M6P) receptors and synthesize IGF-II: autocrine growth stimulation by IGF-II via the IGF-I receptor. J. Cell. Physiol. 159 : 531-541 , 1994.