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脫氧核糖核酸酶Ⅱ 來源于豬脾, ≥800 units/mg dry weight
??【編號(hào)】:128600

??【產(chǎn)品名稱】:脫氧核糖核酸酶Ⅱ 來源于豬脾, ≥800 units/mg dry weight

??【規(guī)格】:80KU

??【用途】:

  脫氧核糖核酸酶Ⅱ 來源于豬脾, ≥800 units/mg dry weight

  Product Name: Deoxyribonuclease II from porcine spleen
  CAS號(hào):9025-64-3
  介紹: Deoxyribonuclease II from porcine spleen has a molecular weight of 38 kDa. The enzyme is a glycoprotein endonuclease with dimeric structure. Optimum pH range is 4.5-5.0 at ionic strength 0.15 M. Deoxyribonuclease II (Acid DNase) hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3'-phosphates. It also acts on p-nitrophenylphosphodiesters at pH 5.6-5.9. Bernardi (BBRC, 17, 573, 1971) describes a three stage degradation of native DNA by DNase II.
  用途: DNase II has been used to treat transformed cells during the purification of β-lactamase. It has also been used for the preparation of adenoma tissue in a study that investigated the effect of somatoprim on growth hormone secretion in human adenoma cell cultures (hSA). Deoxyribonuclease II from bovine spleen has been used in a study that conducted a partial purification of deoxyribonucleases from eggs and liver of Xenopus laevis. Deoxyribonuclease II from bovine spleen has also been used in a study to investigate nucleic acid and protein synthesis of splenic lymphocytes.
  貯存: 儲(chǔ)存溫度2-8℃
  生化和生理學(xué)機(jī)理: The enzyme is a lysosomal acid hydrolase that hydrolyzes DNA to 3′-phosphoryl oligonucleotides in the absence of metal ions, under acidic conditions. It is a non-covalently linked α·β heterodimer of approximately 45,000 Da molecular mass.
上一篇:DNA酶I 來源于牛胰腺(過濾), ≥2,000 units/mg dry weight 下一篇:氧核糖核酸酶Ⅱ 來源于豬脾(純化), ≥12,000 units/mg protein



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