5,5＇二硫代雙(2-硝基苯甲酸), 98%

??【編號】：D105559

??【產(chǎn)品名稱】：5,5＇二硫代雙(2-硝基苯甲酸), 98%

??【規(guī)格】：1g

??【用途】：

　　5,5＇二硫代雙(2-硝基苯甲酸), 98%

　　Product Name： 5,5＇-Dithio bis-(2-nitrobenzoic acid)

　　別名：雙(3-羧基-4-硝基苯基)二硫，6,6ˊ-二硝基-3,3ˊ-二硫代苯甲酸 ;DTNB,Ellman’s Reagent;二硫代硝基苯甲酸

　　CAS號：69-78-3

　　分子式：C14H8N2O8S2

　　分子量：396.35

　　介紹： Ellman's Reagent (DTNB) reacts with sulfhydryl groups to yield a colored product, providing a reliable method to measure reduced cysteines and other free sulfhydryls in solution. Ellman's Reagent (5,5'-dithio-bis-[2-nitrobenzoic acid]) is used to estimate sulfhydryl groups in a sample by comparing to a standard curve of a sulfhydryl-containing compound such as cysteine. Alternatively, sulfhydryl groups can be assayed using the extinction coefficient of TNB (14,150M-1cm-1 at 412nm). Ellman's Reagent has been used for applications other than sulfhydryl estimation, such as for determining alkylthiols by HPLC using a pre-column derivatization procedure and to study thiols in the active site of several enzymes. Highlights： ?Sulfhydryl assay – reacts quantitatively with free (reduced) sulfhydryl groups (–SH) to yield detectable TNB product ?Colorimetric detection – colored product enables spectrophotometric measurement in cuvettes or microplates (λmax = 412nm; ε = 14,150/M?cm) ?Proven method – well-characterized chemistry provides quantitation of peptide or protein sulfhydryl groups using extinction coefficient calculation or by comparison to a cysteine standard

　　用途：巰基測定，是測定蛋白質(zhì)、多肽和組織中巰基的靈敏試劑。也用于有機磷農(nóng)藥中毒的監(jiān)測（膽堿酯酶測定）。

　　熔點： 240～245℃

　　貯存：陰涼干燥處保存;儲存溫度2-8°C

　　敏感性：熱敏感

上一篇：2,4-二氯苯氧乙酸, 97%		下一篇：脫氫乙酸, 98%

5,5＇二硫代雙(2-硝基苯甲酸), 98%

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5,5＇二硫代雙(2-硝基苯甲酸), 98%

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　　5,5＇二硫代雙(2-硝基苯甲酸), 98%

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