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白介素18（人類rDNA衍生）（WHO參考試劑）
??【編號】：NIBSC03/200

??【名稱】：白介素18（人類rDNA衍生）（WHO參考試劑）

??【規(guī)格】：1ml

??【價(jià)格】：2600元

　　Interleukin - 18 (Human rDNA derived)(WHO Reference Reagent)標(biāo)準(zhǔn)品

　　編號：NIBSC03/200
　　中文：白介素18
　　規(guī)格：1ml
　　描述：白細(xì)胞介素18（人類rDNA衍生）（WHO參考試劑）
　　類型：國際參考試劑
　　描述：1. INTENDED USE
This standard is the primary biological standard fort Interleukin-18 (IL-18).
2. CAUTION
This preparation is not for administration to humans or animals in
the human food chain.
The preparation contains material of human origin, and either the final
product or the source materials, from which it is derived, have been
tested and found negative for HBsAg, anti-HIV and HCV RNA. As with
all materials of biological origin, this preparation should be regarded as
potentially hazardous to health. It should be used and discarded
according to your own laboratory's safety procedures. Such safety
procedures should include the wearing of protective gloves and
avoiding the generation of aerosols. Care should be exercised in
opening ampoules or vials, to avoid cuts.
3. UNITAGE
In a limited collaborative study, the preparation coded 03/200 was found
to possess IL-18 activity comparable to in-house standards, based on its
ability to stimulate release of IFN-gamma from a human myelomonocytic
cell line. The preparation is therefore established as the WHO Reference
Reagent for IL-18 for bioassay, with an assigned potency of 10,000 Units
per ampoule. It should be noted that this assigned potency is arbitrary,
and does not reflect any pre-existing units for IL-18 activity, and it does not
establish continuity with any pre-existing in-house standards. Users of the
material should also note that the preparation 03/200 may be suitable to
serve as a standard for the bioassay of IL-18 using assay methods based
on other biological activities of IL-18, but such applications have not been
demonstrated for 03/200, and would require appropriate validation. Users
who have data supporting the use of this preparation in another biological
assay are encouraged to contact NIBSC.
The preparation 03/200 was also shown to possess immunoactivity
comparable to in-house IL-18 standards, in a limited number of in-house
immunoassay systems. Whilst 03/200 is not formally established as an
immunoassay standard, it may be suitable as an immunoassay standard,
subject to appropriate validation. Users should note particularly that the
immunological stability of this preparation, stability after freezing and
thawing of the reconstituted ampoule contents has not been
demonstrated for all immunoassay systems, and such studies should be
undertaken by the user as part of such immunoassay application
validation.
4. CONTENTS
Country of origin of biological material: United Kingdom.
Each ampoule contains the residue after freeze-drying of 1.0 ml of a
solution comprising:
IL-18 approximately 1 microgram
9.0 mg sodium chloride
1.0 mg trehalose
2.0 mg human serum albumin
0.1 microlitre Tween 20
The IL-18 protein was expressed in E.coli
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