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白介素12（人類，rDNA衍生）世衛(wèi)組織參考試劑
??【編號】：NIBSC95/544

??【名稱】：白介素12（人類，rDNA衍生）世衛(wèi)組織參考試劑

??【規(guī)格】：1個

??【價格】：2600元

　　白介素12（人類，rDNA衍生）世衛(wèi)組織參考試劑

　　編號：NIBSC95/544
　　英文：Interleukin-12 (Human, rDNA derived) WHO Reference Reagent
　　標(biāo)準(zhǔn)類型：國際參考試劑
　　規(guī)格：1個 
　　描述：1. INTENDED USE
This Standard is the primary biological standard for IL-12.
2. CAUTION
This preparation is not for administration to humans or animals in
the human food chain.
The preparation contains material of human origin, and either the final
product or the source materials, from which it is derived, have been
tested and found negative for HBsAg, anti-HIV and HCV RNA. As with
all materials of biological origin, this preparation should be regarded as
potentially hazardous to health. It should be used and discarded
according to your own laboratory's safety procedures. Such safety
procedures should include the wearing of protective gloves and
avoiding the generation of aerosols. Care should be exercised in
opening ampoules or vials, to avoid cuts.
3. UNITAGE
The assigned potency is 10,000 Units of biological activity per ampoule.
4. CONTENTS
Country of origin of biological material: United Kingdom.
Each ampoule contains a freeze dried residue comprising, under an
atmosphere of nitrogen:
IL-12 approximately 1 microgram
Potassium phosphate buffer
1.0 mg trehalose
2.0 mg human serum albumin
The IL-12 protein was expressed in CHO cells.
5. STORAGE
For economy of use, it is recommended that the solution be sub
divided into several small aliquots and stored at -40ºC or below.
Avoid repeated thawing/freezing. Unopened ampoules should be
stored at -20ºC.
Please note: because of the inherent stability of lyophilized
material, NIBSC may ship these materials at ambient temperature.
6. DIRECTIONS FOR OPENING
DIN ampoules have an 'easy-open' coloured stress point, where the
narrow ampoule stem joins the wider ampoule body. Various types of
ampoule breaker are available commercially. To open the ampoule,
tap the ampoule gently to collect material at the bottom (labelled) end
and follow manufactures instructions provided with the ampoule
breaker.
7. USE OF MATERIAL
No attempt should be made to weigh out any portion of the freeze-dried
material prior to reconstitution
Dissolve the total contents of the ampoule in 0.5ml of sterile distilled
water. Rinse the ampoule with about 0.4ml of sterile distilled water and
make up the total volume to 1.0ml with distilled water. This solution will
contain IL-12 at a concentration of 10000 Units/ml. Use carrier protein
where extensive dilution is required.
8. STABILITY
Reference materials are held at NIBSC within assured, temperaturecontrolled
storage facilities and they should be stored on receipt as
indicated on the label. It is the policy of WHO not to assign an expirydate to their international reference materials. Accelerated degradation
studies have indicated that this material is suitably stable, when stored at
-20ºC or below, for the assigned values to remain valid until the material
is withdrawn or replaced. These studies have also shown that the
material is suitably stable for shipment at ambient temperature without
any effect on the assigned values. Once reconstituted, diluted or
aliquoted, users should determine the stability of the material according
to their own method of preparation, storage and use. Users who have
data supporting any deterioration in the characteristics of any reference
preparation are encouraged to contact NIBSC.
9. REFERENCES
This standard was produced under WHO guidelines cited in the WHO
Technical Reports Series, No. 800, 1990, Annex 4.
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