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白介素3（人類，rDNA衍生）世衛(wèi)組織國(guó)際標(biāo)準(zhǔn)
??【編號(hào)】：NIBSC91/510

??【名稱】：白介素3（人類，rDNA衍生）世衛(wèi)組織國(guó)際標(biāo)準(zhǔn)

??【規(guī)格】：1個(gè)

??【價(jià)格】：2600元

　　白介素3（人類，rDNA衍生）世衛(wèi)組織國(guó)際標(biāo)準(zhǔn)

　　編號(hào)：NIBSC91/510
　　英文：Interleukin-3 (Human, rDNA derived) WHO International Standard
　　標(biāo)準(zhǔn)類型： 國(guó)際標(biāo)準(zhǔn)
　　規(guī)格：1個(gè) 
　　描述：1. INTENDED USE
This International Standard is the primary biological standard for IL3.
2. CAUTION
This preparation is not for administration to humans or animals in
the human food chain.
The preparation contains material of human origin, and either the final
product or the source materials, from which it is derived, have been
tested and found negative for HBsAg, anti-HIV and HCV RNA. As with
all materials of biological origin, this preparation should be regarded as
potentially hazardous to health. It should be used and discarded
according to your own laboratory's safety procedures. Such safety
procedures should include the wearing of protective gloves and
avoiding the generation of aerosols. Care should be exercised in
opening ampoules or vials, to avoid cuts.
3. UNITAGE
The assigned potency agreed on the basis of WHO International
Collaborative Study is 1700 International Units of biological activity per
ampoule.
4. CONTENTS
Country of origin of biological material: United Kingdom.
Each ampoule contains a freeze dried residue comprising, under an
atmosphere of nitrogen:
IL-3 approximately 1.0 microgram
4.5 mg sodium chloride
0.5 mg trehalose
1.0 mg human serum albumin
The IL-3 protein was expressed in E. coli.
5. STORAGE
For economy of use, it is recommended that the solution be sub divided
into several small aliquots and stored at -40ºC or below. Avoid repeated
thawing/freezing. Unopened ampoules should be stored at -20ºC.
Please note: because of the inherent stability of lyophilized
material, NIBSC may ship these materials at ambient temperature.
6. DIRECTIONS FOR OPENING
Tap the ampoule gently to collect the material at the bottom (labelled)
end. Ensure ampoule is scored all round at the narrow part of the
neck, with a diamond or tungsten carbide tipped glass knife file or
other suitable implement before attempting to open. Place the
ampoule in the ampoule opener, positioning the score at position 'A';
shown in the diagram below. Surround the ampoule with cloth or
layers of tissue paper. Grip the ampoule and holder in the hand and
squeeze at point 'B'. The ampoule will snap open. Take care to avoid
cuts and projectile glass fragments that enter eyes. Take care that no
material is lost from the ampoule and that no glass falls into the
ampoule.Side view of ampoule opening device containing an ampoule positioned
ready to open. 'A' is the score mark and 'B' the point of applied pressure.
7. USE OF MATERIAL
No attempt should be made to weigh out any portion of the freeze-dried
material prior to reconstitution
Dissolve the total contents of the ampoule in 0.5ml of sterile distilled water.
Rinse the ampoule with about 0.4ml of sterile phosphate buffered saline
(PBS) and make up the total volume to 1.0ml with PBS. This solution will
contain IL-3 at a concentration of 1700 Units/ml. Use carrier protein where
extensive dilution is required.
8. STABILITY
Reference materials are held at NIBSC within assured, temperaturecontrolled
storage facilities and they should be stored on receipt as
indicated on the label. It is the policy of WHO not to assign an expiry date
to their international reference materials. Accelerated degradation studies
have indicated that this material is suitably stable, when stored at -20ºC
or below, for the assigned values to remain valid until the material is
withdrawn or replaced. These studies have also shown that the material
is suitably stable for shipment at ambient temperature without any effect
on the assigned values. Once reconstituted, diluted or aliquoted, users
should determine the stability of the material according to their own
method of preparation, storage and use. Users who have data
supporting any deterioration in the characteristics of any reference
preparation are encouraged to contact NIBSC.
9. REFERENCES
Mire-Sluis, A. R, Gaines Das, R., Thorpe, R. et al (1996) Implications for the
assay and biological properties of interleukin-3. Results of a WHO
international collaborative study. Journal of Immunological Methods 194, 1-
12.
This standard was produced under WHO guidelines cited in the WHO
Technical Reports Series, No. 800, 1990, Annex 4.
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